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AMPK alpha Antibody

Catalog No : B0003
  • Applications
    WB, IHC, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-AMPK alpha Antibody detects endogenous levels of total AMPK alpha protein. Validated for WB, IHC, and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    AMPK alpha Antibody
  • Catalog No
    B0003
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
  • Database Links
    Gene ID: 5562/5563, Swiss-Prot #: Q13131/P54646, OMIM #: 602739/600497, Unigene #: Hs.43322/Hs.437039
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human AMPK alpha.
  • Immunogen Range
    140-189
  • MW
    62 kDa
  • NCBI Gene Symbol
    AAPK1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
  • Specificity
    AMPK alpha Antibody detects endogenous levels of total AMPK alpha protein.
  • Target Modification
    Unmodified/Total
Application Images
Image 1 Western blot analysis of lysates from COS7 cells, treated with Adriamycin 0.5ng/ml 24h, using AMPK alpha Antibody. The lane on the right is blocked with the synthesized peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using AMPK alpha Antibody. The picture on the right is blocked with the synthesized peptide.
Image 3 Western Blot analysis of various cells using AMPKalpha1/2 Antibody diluted at 1:500
Image 4 Western Blot analysis of COS7 cells using AMPKalpha1/2 Antibody diluted at 1:500
Image 5 Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. AMPKalpha1/2 Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.
Image 6 Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. AMPKalpha1/2 Antibody was diluted at 1:200(4°C,ON). Negative control was defined by secondary antibody only.