Anti-Amyloid beta A4 (Phospho-Thr743/668) polyclonal Antibody detects Amyloid beta A4 only when phosphorylated at Thr743/668. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
Amyloid beta A4 (Phospho-Thr743/668) Antibody
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Catalog No
A0004
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, IF, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
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Database Links
Gene ID: 351, Swiss-Prot #: P05067, OMIM #: 104300/104760/605714/609065, Unigene #: Hs.434980
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Immunogen
The antiserum was produced against synthesized peptide derived from human Amyloid beta A4 around the phosphorylation site of Thr743/668.
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Immunogen Range
711-760
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Modification Sites
H: T743 M: T743 R: T743
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MW
86 kDa
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NCBI Gene Symbol
APP
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
Amyloid beta A4 (Phospho-Thr743/668) Antibody detects endogenous levels of Amyloid beta A4 only when phosphorylated at Thr743/668.
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from HeLa cells, using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Amyloid-beta (T743) Antibody
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from HeLa cells, using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human brain, using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The picture on the right is blocked with the phospho peptide. |
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Immunofluorescence analysis of HeLa cells, using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-Amyloid-beta (T743) Antibody |
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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