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AMPKalpha (Phospho-Thr172) Colorimetric Cell-Based ELISA Kit

Catalog No : CBP1003
  • Reactivity
    Human, Mouse, Rat
  • Size
    3+ Kits
  • Price
    Inquire
The AMPKalpha (Phospho-Thr172) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect AMPKalpha protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated AMPKalpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on AMPKalpha phosphorylation.
Details
  • Product Name
    AMPKalpha (Phospho-Thr172) Colorimetric Cell-Based ELISA Kit
  • Catalog No
    CBP1003
  • Detection Method
    Colorimetric 450 nm
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based
  • Database Links
    Gene ID: 5562/5563, UniProt ID: Q13131/P54646, OMIM #: 602739/600497, Unigene #: Hs.43322/Hs.437039
  • Format
    2 x 96-Well Microplates
  • Manual (PDF)
  • NCBI Gene Symbol
    AAPK1/AAPK2
  • Sub Type
    Phospho
  • TargetName
    AMPKalpha (Phospho-Thr172)
Application Images
Image 1 Western blot analysis of extracts from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody.
Image 2 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 3 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 4 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
References
This product has been referenced in:
  • Nathan P. Ward, Angela M. Poff, Andrew P. Koutnik, Dominic P. D’Agostino "Complex I inhibition augments dichloroacetate cytotoxicity through enhancing oxidative stress in VM-M3 glioblastoma cells " PLOS One ELISA Read more >