The ADD1/2 (Phospho-Ser726) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect ADD1/2 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated ADD1/2 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ADD1/2 phosphorylation.
Details
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Product Name
ADD1/2 (Phospho-Ser726) Colorimetric Cell-Based ELISA Kit
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Catalog No
CBP1002
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Detection Method
Colorimetric 450 nm
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Dynamic Range
> 5000 Cells
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Storage/Stability
4°C/6 Months
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Reactivity
Human, Mouse, Rat
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Assay Type
Cell-Based
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Database Links
Gene ID: 118/119, UniProt ID: P35611/P35612, OMIM #: 102680/102681, Unigene #: Hs.183706/Hs.701490/Hs.188528
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Format
2 x 96-Well Microplates
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Manual (PDF)
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NCBI Gene Symbol
ADD1/ADD2
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Sub Type
Phospho
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Synonyms
Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA
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TargetName
ADD1/2 (Phospho-Ser726)
Application Images
Western blot analysis of extracts from HeLa cells treated with Forskolin 40nM 30', using ADD1 (Phospho-Ser726) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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Western blot analysis of extracts from HeLa cells treated with Forskolin 40nM 30', using ADD1 (Phospho-Ser726) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |
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