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14-3-3 zeta (Phospho-Ser58) Colorimetric Cell-Based ELISA Kit

Catalog No : CBP1001
  • Reactivity
    Human, Mouse, Rat
  • Size
    3 Kits
  • Price
    $1,440.00
The 14-3-3 zeta (Phospho-Ser58) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect 14-3-3 zeta protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated 14-3-3 zeta in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on 14-3-3 zeta phosphorylation.
Details
  • Product Name
    14-3-3 zeta (Phospho-Ser58) Colorimetric Cell-Based ELISA Kit
  • Catalog No
    CBP1001
  • Detection Method
    Colorimetric 450 nm
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based
  • Database Links
    Gene ID: 7534, UniProt ID: P63104, OMIM #: 601288, Unigene #: Hs.492407/Hs.594673
  • Format
    2 x 96-Well Microplates
  • Manual (PDF)
  • NCBI Gene Symbol
    YWHAZ
  • Sub Type
    Phospho
  • Synonyms
    14-3-3 protein zeta/delta, 1433Z, 143Z, FAS, Factor activating exoenzyme S, KCIP-1, Mitochondrial import stimulation factor S1 subunit, Protein kinase C inhibitor protein-1, YWHAZ
  • TargetName
    14-3-3 zeta (Phospho-Ser58)
Application Images
Image 1 Western blot analysis of extracts from NIH-3T3 cells treated with UV 30', using 14-3-3 zeta (Phospho-Ser58) Antibody.
Image 2 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 3 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 4 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.