The Tfact c-Jun (Phospho-Ser249) DNA-Binding ELISA Kit components necessary for detection and qualitative analysis of c-Jun (Phospho-Ser249) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
Details
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Product Name
TFact c-Jun (Phospho-Ser249) DNA-Binding ELISA
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Catalog No
TFE-7005
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Detection Method
Colorimetric 450 nm
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Storage/Stability
4°C/6 Months
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Reactivity
Human, Mouse, Rat
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Assay Type
DNA-Binding ELISA
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Database Links
Gene ID: 3725, UniProt ID: P05412, OMIM #: 165160, Unigene #: Hs.714791
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Format
12 x 8-Well Microstrips
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Image Legend
The TFact™, c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.
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Manual (PDF)
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NCBI Gene Symbol
JUN
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Specificity
The TFact™, c-Jun (Phospho-Ser249) DNA-Binding ELISA Kit detects endogenous levels of c-Jun only when phosphorylated at Ser249.
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Sub Type
Phospho
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Synonyms
Activator protein 1, AH119, AP-1, AP1, c-Jun, Jun A, p39, Proto-oncogene c-jun, RJG-9, V-jun avian sarcoma virus 17 oncogene homolog
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TargetName
c-Jun (Phospho-Ser249)
Application Images
The Tfact c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest.
An illustrated breakdown of Assay Biotechnology's Colorimetric Transcription Factor ELISA assay principles.
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The Tfact c-Jun DNA-Binding ELISA kits detect active c-Jun in Hela Nuclear Extract. The Hela cells were grown 3 days in DMEM with 10% FBS and harvested for nuclear extract. The Hela cells were stimulated by UV (100J/M2) before harvest. |
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An illustrated breakdown of Assay Biotechnology's Colorimetric Transcription Factor ELISA assay principles. |
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