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AMPKalpha (Phospho-Thr172) Fluorometric Cell-Based ELISA Kit

Catalog No : FLUO-CBP1003
  • Reactivity
    Human, Mouse, Rat
  • Size
    3 Kits
  • Price
    Inquire
The AMPKalpha (Phospho-Thr172) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor AMPK? (Phospho-Thr172) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated AMPK? (Phospho-Thr172) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on AAPK1/AAPK2 phosphorylation.
Details
  • Product Name
    AMPKalpha (Phospho-Thr172) Fluorometric Cell-Based ELISA Kit
  • Catalog No
    FLUO-CBP1003
  • Detection Method
    Fluorometric
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 5562/5563, UniProt ID: Q13131/P54646, OMIM #: 602739/600497, Unigene #: Hs.43322/Hs.437039
  • Format
    Two 96-Well Plates
  • Manual (PDF)
  • NCBI Gene Symbol
    AAPK1/AAPK2
  • Sub Type
    Phospho
  • TargetName
    Phospho-AMPKα (Thr172)
Application Images
Western blot analysis of extracts from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody.
HeLa cells starved for 24 hrs. and stimulated with LPS 100ng/ml 2 hrs.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
Western blot analysis of extracts from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody.
HeLa cells starved for 24 hrs. and stimulated with LPS 100ng/ml 2 hrs.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.