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Fluorescent 488 tyramide

Catalog No : Z1508
  • Size
    200 Slides
  • Price
For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. Fluorescent 488 tyramide contains the extremely bright and photostable Fluorescent 488 that can be readily detected with the standard FITC filter set.
  • Product Name
    Fluorescent 488 tyramide
  • Catalog No
  • ABT-Webpage
  • H-Phrases
    H303, H313, H333
  • Molecular weight
  • Product Legend
    Immunofluorescent image of paraffin-embedded human lung carcinoma labeled with?EpCAM Rabbit mAb followed with HRP-labeled goat anti-rabbit IgG (H+L) . The signal was developed with Fluorescent 488 tyramide or Alexa Fluorescent 488 Tyramide Reagent (Green). Cells were also counterstained with DAPI (Blue).
  • R-Phrase
    R20, R21, R22
  • Shipping
  • Solvent
  • Spectral Properties
    Excitation (nm):491, Emission (nm):516, Extinction coefficient (cm-1 M-1):75000, Quantum yield:0.9
  • Storage
  • Symbol
  • UNSPEC Code
Application Images