BOC-Val-Pro-Arg-AMC is a sensitive fluorogenic substrate for alpha-thrombin. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has an emission spectra that can be easily detected with the DAPI filter set. The Km values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 21 and 25 uM respectively. The Kcat values of alpha-thrombin and the alpha-thrombin-staphylocoagulase complex for BOC-Val-Pro-Arg-AMC were reported to be 105 and 89 s-1 respectively. This substrate has also been used for the rapid detection of MRSA (methicillin-resistant Staphylococcus aureus).
Details
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Product Name
BOC-Val-Pro-Arg-AMC
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Catalog No
Z0068
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ABT-Webpage
assaybiotechnology.com/BOC-Val-Pro-Arg-AMC
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H-Phrases
H303, H313, H333
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Molecular weight
741.75
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Product Legend
Chemical structure for BOC-Val-Pro-Arg-AMC
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R-Phrase
R20, R21, R22
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Shipping
RT
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Solvent
DMSO
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Spectral Properties
Excitation (nm):341, Emission (nm):441
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Storage
f/l
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Symbol
Xn
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UNSPEC Code
12352200
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