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Fluorescent 488 hydrazide

Catalog No : Z0839
  • Size
    1 mg
  • Price
    $205.00
Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with Fluorescent 488 dyes are far superior compared to conjugates of fluorescein derivatives such as FITC. Fluorescent 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of Fluorescent 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. Fluorescent 488 hydrazide is reasonably stable and shows good reactivity and selectivity with carbonyl group (such as aldehyde). This Fluorescent 488 has spectral properties and reactivity similar to Alexa Fluor? 488 hydrazide ( Alexa Fluor? is the trademark of Invitrogen).
Details
  • Product Name
    Fluorescent 488 hydrazide
  • Catalog No
    Z0839
  • ABT-Webpage
    assaybiotechnology.com/Fluorescent-488-hydrazide
  • H-Phrases
    H303, H313, H333
  • Molecular weight
    890.57
  • Product Legend
    Fluorescent dye hydrazine derivatives are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester). Fluorescent dye hydrazine derivatives are also useful for tracing neurons.
  • R-Phrase
    R20, R21, R22
  • Shipping
    RT
  • Solvent
    Water
  • Spectral Properties
    Excitation (nm):491, Emission (nm):516, Extinction coefficient (cm-1 M-1):75000, Quantum yield:0.9
  • Storage
    f/l
  • Symbol
    Xn
  • UNSPEC Code
    12171501
Application Images