Fluorescamine is a popular amine-fluorogenic reagent for determining protein concentrations in solutions and on gels. It is also used to analyze low molecular weight amines by TLC, HPLC and capillary electrophoresis. Fluorescamine is nonfluorescent but readily reacts with primary aliphatic amines, including those in peptides and proteins, to yield a strongly blue-green fluorescent adduct that can be excited by UV light. The amine adduct has absorption maximum at 385 nm and fluorescence maximum at 486 nm.
Details
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Product Name
Fluorescamine CAS 38183-12-9
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Catalog No
Z0146
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ABT-Webpage
assaybiotechnology.com/Fluorescamine-CAS-38183-12-9
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H-Phrases
H303, H313, H333
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Molecular weight
278.26
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Product Legend
Chemical structure for Fluorescamine *CAS 38183-12-9*
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R-Phrase
R20, R21, R22
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Shipping
RT
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Solvent
DMSO
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Spectral Properties
Excitation (nm):382, Emission (nm):480, Absorbance (nm):237
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Storage
f/l
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Symbol
Xn
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UNSPEC Code
12171501
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