Fluorescent dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). Fluorescent 350 dyes have fluorescence excitation and emission maxima of ~350 nm and ~440 nm respectively. These spectral characteristics make them an excellent alternative to AMCA and Alexa Fluor? 350 labeling dye (Alexa Fluor? is the trademark of Invitrogen). Fluorescent 350 maleimide is reasonably stable and shows good reactivity and selectivity with thiol group.
Fluorescent 350 maleimide
H303, H313, H333
HeLa cells were stained with mouse anti-tubulin followed by Fluorescent 350 goat anti-mouse IgG (H+L).
R20, R21, R22
Excitation (nm):343, Emission (nm):441, Extinction coefficient (cm-1 M-1):19000, Correction factor (260 nm):0.25, Correction factor (280 nm):0.19