(TF8WS) family has the spectral properties similar to those of IRDye 800. Their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family more robust to pH-sensitive assays. In some cases TF7-labeled peptides and nucleotides exhibit stronger fluorescence and higher photostability than the ones labeled with IRDye 800. This TF8WS product is reactive to alkynes, and useful for click chemistry.
TFluorophore 8WS azide [TF8WS azide]
H303, H313, H333
Click chemistry is a method for attaching a?probe?or?substrate?of interest to a specific biomolecule, a process called?bioconjugation. The possibility of attaching?fluorophores?and other?reporter molecules?has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an?azide?with an?alkyne?to form a 5-membered?heteroatom?ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne?Cycloaddition?(CuAAC).
R20, R21, R22
Excitation (nm):785, Emission (nm):801, Extinction coefficient (cm-1 M-1):250000