(TF6WS) family has the spectral properties similar to those of Cy5.5, IRDye 700 and Alexa Fluor 680. Their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family more robust to pH-sensitive assays. In some cases TF6-labeled peptides and nucleotides exhibit stronger fluorescence and higher photostability than the ones labeled with Cy5.5, IRDye 700 and Alexa Fluor 680. This TF6WS product is is used for post-labeling of thiol-modified oligonucleotides and peptides that contain cysteines.
TFluorophore 6WS maleimide [TF6WS maleimide]
H303, H313, H333
Fluorescent dye maleimides are the most popular tool for conjugating dyes to a peptide, protein, antibody, thiol-modified oligonucleotide or nucleic acid through their SH group. Maleimides react readily with the thiol group of proteins, thiol-modified oligonucleotides, and other thiol-containing molecules under neutral conditions. The resulting dye conjugates are quite stable.
R20, R21, R22
Excitation (nm):682, Emission (nm):701, Extinction coefficient (cm-1 M-1):220000