Cationic Dye Blue is a cationic dye that selectively accumulates in mitochondria probably via the mitochondrial membrane potential gradient. The mitochondrial indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in mitochondria after it gets into cells. This fluorescent mitochondrial indicator is retained in mitochondria for long time since the indicator carries a cell-retaining group. This key feature significantly increases its staining efficiency. The labeling protocol is robust, requiring minimal hands-on time. It can be readily adapted for a wide variety of fluorescence platforms such as microplate assays, immunocytochemistry and flow cytometry. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells.
Cationic Dye Blue FX490
H303, H313, H333
Image of HeLa cells stained with the Cationic Dye Blue FX490 in a 96-well clear-bottom plate.
R20, R21, R22
Excitation (nm):344, Emission (nm):469