Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris. Lysosomes digest excess or worn-out organelles, food particles, and engulfed viruses or bacteria. The membrane around a lysosome allows the digestive enzymes to work at pH 4.5. The interior of the lysosomes is acidic (pH 4.5-4.8) compared to the slightly alkaline cytosol (pH 7.2). The lysosome maintains this pH differential by pumping protons from the cytosol across the membrane via proton pumps and chloride ion channels. Lysosome Dye Deep Red selectively accumulates in lysosomes probably via the lysosome pH gradient. The lysotropic indicator is a hydrophobic compound that easily permeates intact live cells, and trapped in lysosomes after it gets into cells. Its fluorescence is significantly enhanced upon entering lysosomes. This key feature significantly reduces its staining background and makes it useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. It is suitable for proliferating and non-proliferating cells, and can be used for both suspension and adherent cells. Lysosome Dye dyes significantly outperform the equivalent LysoTracker dyes (from Invitrogen). Lysosome Dye dyes can stay in live cells for more than a week with very minimal cell toxicity while the LysoTracker dyes can only be used for a few hours. Lysosome Dye dyes can survive a few generations of cell division. In addition, Lysosome Dye dyes are much more photostable than the LysoTracker dyes.
Lysosome Dye Deep Red
H303, H313, H333
Image of Hela cells stained. The TRTIC signals were compared at 0 and 120 seconds exposure time by using an Olympus fluorescence microscope.
R20, R21, R22
Excitation (nm):597, Emission (nm):619