(TF3) family has the spectral properties essentially identical to those of Cy3. Compared to Cy3 probes TF3 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to Cy3. TF3-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with Cy3. This TF3 product is reactive to alkynes, and useful for click chemistry.
TFluorophore 3 azide [TF3 azide]
H303, H313, H333
Click chemistry is a method for attaching a?probe?or?substrate?of interest to a specific biomolecule, a process called?bioconjugation. The possibility of attaching?fluorophores?and other?reporter molecules?has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an?azide?with an?alkyne?to form a 5-membered?heteroatom?ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne?Cycloaddition?(CuAAC).
R20, R21, R22
Excitation (nm):546, Emission (nm):571, Extinction coefficient (cm-1 M-1):85000