(TF2) family has the spectral properties similar to those of fluoresceins and Alexa Fluor? 488 (Alexa Fluor? is the trademark of Invitrogen). TF2 phosphoramidite enables the oligo labeling with the similar spectral properties and photostability of Alexa Fluor? 488 using the well established phosphoramidite chemistry. Compared to fluoresceins TF2 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to fluoresceins. TF2-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with FITC or FAM. In addition, TF2 is an excellent replacement for the expensive Alexa Fluor? 488 due to their similar spectral properties, pH dependence and photostability.
Details
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Product Name
TFluorophore 2 alkyne [TF2 alkyne]
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Catalog No
Z1004
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ABT-Webpage
assaybiotechnology.com/TFluorophore-2-alkyne-TF2-alkyne
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H-Phrases
H303, H313, H333
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Molecular weight
752.61
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Product Legend
Click chemistry is a method for attaching a?probe?or?substrate?of interest to a specific biomolecule, a process called?bioconjugation. The possibility of attaching?fluorophores?and other?reporter molecules?has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an?azide?with an?alkyne?to form a 5-membered?heteroatom?ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne?Cycloaddition?(CuAAC).
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R-Phrase
R20, R21, R22
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Shipping
RT
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Solvent
DMSO
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Spectral Properties
Excitation (nm):503, Emission (nm):525, Extinction coefficient (cm-1 M-1):75000
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Storage
f/l
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Symbol
Xn
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UNSPEC Code
12171501
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