(TF2) family has the spectral properties similar to those of fluoresceins and Alexa Fluor? 488 (Alexa Fluor? is the trademark of Invitrogen). TF2 phosphoramidite enables the oligo labeling with the similar spectral properties and photostability of Alexa Fluor? 488 using the well established phosphoramidite chemistry. Compared to fluoresceins TF2 family has much stronger fluorescence and higher photostability. Additionally their fluorescence is pH-independent from pH 3 to 11. These characteristics make this new dye family a superior alternative to fluoresceins. TF2-labeled peptides and nucleotides exhibit much stronger fluorescence and higher photostability than the ones labeled with FITC or FAM. In addition, TF2 is an excellent replacement for the expensive Alexa Fluor? 488 due to their similar spectral properties, pH dependence and photostability. This TF2 product is reactive to alkynes, and useful for click chemistry.
TFluorophore 2 azide [TF2 azide]
H303, H313, H333
Click chemistry is a method for attaching a?probe?or?substrate?of interest to a specific biomolecule, a process called?bioconjugation. The possibility of attaching?fluorophores?and other?reporter molecules?has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an?azide?with an?alkyne?to form a 5-membered?heteroatom?ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne?Cycloaddition?(CuAAC).
R20, R21, R22
Excitation (nm):503, Emission (nm):525, Extinction coefficient (cm-1 M-1):75000