Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the
Nucleotide Dye 535-ddGTP
H303, H313, H333
Chemical structure for Nucleotide Dye 535-ddGTP.
R20, R21, R22
Excitation (nm):503, Emission (nm):536, Absorbance (nm):503