The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This TMR-dUTP conjugate can be used as an orange fluorescence color with Spectrum Orange filter set (Spectrum Orange is the trademark of Vysis).
H303, H313, H333
Chemical structure for Tetramethylrhodamine-dUTP *1 mM in Tris Buffer (pH 7.5)*
R20, R21, R22
Excitation (nm):552, Emission (nm):578, Extinction coefficient (cm-1 M-1):90000