The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This fluorescein-dUTP conjugate can be used as a green fluorescence color with Spectrum Green filter set (Spectrum Green is the trademark of Vysis).
Fluorescein-12-dUTP CAS 214154-36-6
H303, H313, H333
Chemical structure for Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 214154-36-6*
R20, R21, R22
Excitation (nm):498, Emission (nm):517, Absorbance (nm):487, Extinction coefficient (cm-1 M-1):80000, Quantum yield:0.7900, 0.95