The dye-modified deoxyuridine 5'-triphosphates (such as aminoallyl-dUTP) can be used to produce dye-containing DNA by conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. This fluorescein-dUTP conjugate can be used as a green fluorescence color with Spectrum Green filter set (Spectrum Green is the trademark of Vysis).
Details
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Product Name
Fluorescein-12-dUTP CAS 214154-36-6
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Catalog No
Z1598
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ABT-Webpage
assaybiotechnology.com/Fluorescein-12-dUTP-CAS-214154-36-6
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H-Phrases
H303, H313, H333
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Molecular weight
1060.62
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Product Legend
Chemical structure for Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 214154-36-6*
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R-Phrase
R20, R21, R22
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Shipping
Dry Ice
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Solvent
Water
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Spectral Properties
Excitation (nm):498, Emission (nm):517, Absorbance (nm):487, Extinction coefficient (cm-1 M-1):80000, Quantum yield:0.7900, 0.95
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Storage
f/l
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Symbol
Xn
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UNSPEC Code
41116134
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