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AA-UTP [Aminoallyl UTP sodium salt] 4 mM in TE buffer CAS 75221-88-4

Catalog No : Z0132
  • Size
    250 UL
  • Price
    $310.00
The amine-modified uridine 5'-triphosphates (such as aminoallyl-UTP) can be used to produce amine-containing RNA by conventional enzymatic incorporation methods such as transcription, nick translation, random primed labeling, or PCR. Aminoallyl UTP can be readily incorporated into RNA through the conventional enzymatic incorporation techniques. The resulting amine-modified nucleic acids can then be labeled using any of amine-reactive fluorescent dyes, biotins and other amine-reactive reagents. The aminoallyl-modified nucleotides can be incorporated to extremely high and consistent levels compared to the tag-labeled uridine triphosphates that generally have higher stereo-hindrance. Subsequent reaction of the amine-modified nucleic acid with an excess of amine-reactive reagent achieves correspondingly high and consistent labeling efficiencies, regardless of the labeling reagent chosen. This two-step labeling method also eliminates the need to optimize an enzymatic reaction to accommodate different dye-modified nucleotides, which may incorporate at very different rates. This labeling method is widely used for both FISH probes and microarray-based experiments.
Details
  • Product Name
    AA-UTP [Aminoallyl UTP sodium salt] 4 mM in TE buffer CAS 75221-88-4
  • Catalog No
    Z0132
  • ABT-Webpage
    assaybiotechnology.com/AA-UTP-Aminoallyl-UTP-sodium-salt-4-mM-in-TE-buffer-CAS-75221-88-4
  • H-Phrases
    H303, H313, H333
  • Molecular weight
    605.16
  • Product Legend
    Chemical structure for AA-UTP [Aminoallyl UTP sodium salt] *4 mM in TE buffer* *CAS 75221-88-4*
  • R-Phrase
    R20, R21, R22
  • Shipping
    Dry Ice
  • Solvent
    Water
  • Spectral Properties
    n/a
  • Storage
    f/l
  • Symbol
    Xn
  • UNSPEC Code
    41116134
Application Images