Assay Biotech's anti-human secondary antibodies have well-characterized specificity for human immunoglobulins and are useful in the detection,sorting or purification of its specified target. This Fluorescent 405-labeled secondary antibody demonstrated much brighter signal compared to the similar Fluorescent 405 goat anti-human IgG antibodies from other commercial sources,and thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP,AP,fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. This antibody was purified through affinity chromatography and conjugated to Fluorescent 405 (ex/em = 403/427 nm). It is compatible with the 405 nm laser and 450/50 nm bandpass filter (for example,as in the BD FACSAria III).
Fluorescent 405 Goat Anti-human IgG (H+L) Antibody
Excitation (nm):403,Emission (nm):427,Extinction coefficient (cm-1 M-1):37000,Quantum yield:0.91