The p47 phox (Phospho-Ser359) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect p47 phox protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated p47 phox in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on p47 phox phosphorylation.
Details
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Product Name
p47 phox (Phospho-Ser359) Colorimetric Cell-Based ELISA Kit
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Catalog No
CBP1456
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Detection Method
Colorimetric 450 nm
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Dynamic Range
> 5000 Cells
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Storage/Stability
4°C/6 Months
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Reactivity
Human
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Assay Type
Cell-Based
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Database Links
Gene ID: 653361, UniProt ID: P14598, OMIM #: 233700/608512, Unigene #: Hs.647047/Hs.655201
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Format
2 x 96-Well Microplates
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Manual (PDF)
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NCBI Gene Symbol
NCF1
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Sub Type
Phospho
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Synonyms
47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, Neutrophil NADPH oxidase factor 1, P47 phox, p47-phox
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TargetName
p47 phox (Phospho-Ser359)
Application Images
Western blot analysis of extracts from HeLa cells treated with nocodazole 1ug/ml 18h, using p47 phox (Phospho-Ser359) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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Western blot analysis of extracts from HeLa cells treated with nocodazole 1ug/ml 18h, using p47 phox (Phospho-Ser359) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |
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