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p47 phox (Phospho-Ser345) Antibody

Catalog No : A8391
  • Applications
    WB, IHC, ELISA
  • Reactivity
    Human
  • Size
    100ul
  • Price
    $295.00
Anti-p47 phox (Phospho-Ser345) polyclonal Antibody detects p47 phox only when phosphorylated at Ser345. Validated for WB, IHC and ELISA. Reactive for Human. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    p47 phox (Phospho-Ser345) Antibody
  • Catalog No
    A8391
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
  • Database Links
    Gene ID: 653361, Swiss-Prot #: P14598, OMIM #: 233700/608512, Unigene #: Hs.647047/Hs.655201
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human p47 phox around the phosphorylation site of Ser345.
  • Immunogen Range
    311-360
  • Modification Sites
    H: S345
  • MW
    44 kDa
  • NCBI Gene Symbol
    NCF1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    p47 phox (Phospho-Ser345) Antibody detects endogenous levels of p47 phox only when phosphorylated at Ser345.
  • Synonyms
    47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, Neutrophil NADPH oxidase factor 1, P47 phox, p47-phox
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using p47 phox (Phospho-Ser345) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using p47 phox (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of Jurkat cells using Phospho-p47-phox (S345) Antibody diluted at 1:1000
Western blot analysis of 3T3 lysis using Phospho-p47-phox (S345) antibody. Antibody was diluted at 1:1000
Western blot analysis of lysates from HepG2 cells treated with TNF 20ng/ml 5', using p47 phox (Phospho-Ser345) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using p47 phox (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of Jurkat cells using Phospho-p47-phox (S345) Antibody diluted at 1:1000
Western blot analysis of 3T3 lysis using Phospho-p47-phox (S345) antibody. Antibody was diluted at 1:1000
References
This product has been referenced in:
  • View ORCID ProfileChristian Lood, View ORCID ProfileSabine Arve, Jeffrey Ledbetter, View ORCID Pro TLR7/8 activation in neutrophils impairs immune complex phagocytosis through shedding of FcgRIIA jem Primary Antibody doi:10.1002/art.22697CrossRefPubMedGoogle Scholar Read more >
  • Michael A. Rosenbaum, MD,a,b,c Pinaki Chaudhuri, PhD,b Benjamin Abelson, MD,b Brandy N. Cross, MD,b, Apolipoprotein A-I Mimetic Peptide Reverses Impaired Arterial Healing after Injury by Reducing Oxidative Stress NCBIPMC immunoblot analysis "PMCID: PMC4529116 NIHMSID: NIHMS705688 PMID: 26125413 doi: 10.1016/j.atherosclerosis.2015.06.018" Read more >