Anti-p47 phox Antibody detects endogenous levels of total p47 phox protein. Validated for WB, IHC, and ELISA. Reactive for Human. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
p47 phox Antibody
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Catalog No
B1172
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
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Database Links
Gene ID: 653361, Swiss-Prot #: P14598, OMIM #: 233700/608512, Unigene #: Hs.647047/Hs.655201
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Immunogen
The antiserum was produced against synthesized peptide derived from human p47 phox.
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Immunogen Range
331-380
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MW
44 kDa
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NCBI Gene Symbol
NCF1
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
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Specificity
p47 phox Antibody detects endogenous levels of total p47 phox protein.
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Synonyms
47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, Neutrophil NADPH oxidase factor 1, P47 phox, p47-phox
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Target Modification
Unmodified/Total
Application Images
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using p47 phox Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HeLa cells, treated with TSA 400ng/ml 24h, using p47 phox Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using p47 phox Antibody. The picture on the right is blocked with the synthesized peptide. |
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Western blot analysis of lysates from HeLa cells, treated with TSA 400ng/ml 24h, using p47 phox Antibody. The lane on the right is blocked with the synthesized peptide. |
References
This product has been referenced in:
- Do Youn Jun, Hyejin Kim, Won Young Jang, Ji Young Lee, Kiyoshi Fukui, Young Ho Kim "Ectopic overexpression of LAPTM5 results in lysosomal targeting and induces Mcl-1 down-regulation, Bak activation, and mitochondria-dependent apoptosis in human HeLa cells " PLOS One Primary Antibody
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