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p38 MAPK (Phospho-Tyr182) Fluorometric Cell-Based ELISA Kit

Catalog No : FLUO-CBP1641
  • Reactivity
    Human, Mouse, Rat
  • Size
    1 Kit
  • Price
The p38 MAPK (Phospho-Tyr182) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor p38 MAPK (Phospho-Tyr182) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated p38 MAPK (Phospho-Tyr182) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on MAPK14 phosphorylation.
  • Product Name
    p38 MAPK (Phospho-Tyr182) Fluorometric Cell-Based ELISA Kit
  • Catalog No
  • Detection Method
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 1432, UniProt ID: Q16539, OMIM #: 600289, Unigene #: Hs.485233/Hs.588289
  • Format
    Two 96-Well Plates
  • Manual (PDF)
  • NCBI Gene Symbol
  • Sub Type
  • Synonyms
    protein, kinase p38-alpha, MAP kinase MXI2, MAP kinase p38, MAPK14, MAX-interacting protein 2, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38, MK14, MXI2
  • TargetName
    Phospho-p38 MAPK (Tyr182)
Application Images
Image 1 Western blot analysis of extracts from RAW264.7 cells and 293 cells, using p38 MAPK (Phospho-Tyr182) Antibody.
Image 2 HeLa cells were starved for 24 hrs. and stimulated with Etoposide 25uM for 1 hr.
Image 3 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 4 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 5 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.