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Fos TFact™ DNA-Binding ELISA

Catalog No : TFE-7013
  • Reactivity
    Human, Mouse, Rat
  • Size
    2 Kits
  • Price
    $990.00
The TFact Fos DNA-Binding ELISA allows for the detection and qualitative analysis of endogenous levels of activated transcription factors in a variety of nuclear and cell lysates Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
Details
  • Product Name
    Fos TFact™ DNA-Binding ELISA
  • Catalog No
    TFE-7013
  • Detection Method
    Colorimetric 450 nm
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    DNA-Binding ELISA
  • Database Links
    Gene ID: 2353, UniProt ID: P01100, OMIM #: 164810, Unigene #: Hs.25647
  • Format
    12 x 8-Well Microstrips
  • Image Legend
    The TFact™, Fos DNA-Binding ELISA detects active Fos in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated respectively by PMA (200nM) and UV (100J/M2) before harvest. The DNA-transcription factor complex is treated with the stablization buffer in the Jurkat nuclear extract with PMA stimulation.
  • Manual (PDF)
  • NCBI Gene Symbol
    FOS
  • Specificity
    The TFact™, Fos DNA-Binding ELISA Kit detects endogenous levels of total Fos protein.
  • Sub Type
    None
  • Synonyms
    Cellular oncogene fos, FOS, G0/G1 switch regulatory protein 7, G0S7, Proto-oncogene protein c-fos
  • TargetName
    Fos
Application Images
The Tfact Fos DNA-Binding ELISA detects active Fos in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated respectively by PMA (200nM) and UV (100J/M2) before harvest. The DNA-transcription factor complex is treated with the stablization buffer in the Jurkat nuclear extract with PMA stimulation.
An illustrated breakdown of Assay Biotechnology's Colorimetric Transcription Factor ELISA assay principles.
The Tfact Fos DNA-Binding ELISA detects active Fos in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The Jurkat cells were stimulated respectively by PMA (200nM) and UV (100J/M2) before harvest. The DNA-transcription factor complex is treated with the stablization buffer in the Jurkat nuclear extract with PMA stimulation.
An illustrated breakdown of Assay Biotechnology's Colorimetric Transcription Factor ELISA assay principles.