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IkappaB-beta (Phospho-Ser23) Antibody

Catalog No : A0014
  • Applications
    WB, IHC, IF, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Size
    50ul
  • Price
    $195.00
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Details
  • Product Name
    IkappaB-beta (Phospho-Ser23) Antibody
  • Catalog No
    A0014
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Database Links
    Gene ID: 4793, Swiss-Prot #: Q15653, OMIM #: 604495, Unigene #: Hs.9731
  • Image 2
    Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Ser23.
  • Immunogen Range
    8-57
  • Modification Sites
    H: S23 M: S23 R: S23
  • MW
    37 kDa
  • NCBI Gene Symbol
    NFKBIB
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    IkappaB-beta (Phospho-Ser23) Antibody detects endogenous levels of IkappaB-beta only when phosphorylated at Ser23.
  • Synonyms
    I-kappa-B-beta, IKB-B, IKBB, IkappaBbeta, NF-kappa-BIB, NF-kappaB inhibitor beta, NFKBIB, TR- interacting protein 9, TRIP9, Thyroid receptor interacting protein 9
  • Target Modification
    Phospho
Application Images
Image 1 Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 5', using IkappaB-beta (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide.
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