Immunofluorescence analysis of HUVEC cells, using EGFR (Phospho-Thr678) Antibody. The picture on the right is blocked with the phospho peptide.
The antiserum was produced against synthesized peptide derived from human EGFR around the phosphorylation site of Thr678.
H: T678 M: T680 R: T679
NCBI Gene Symbol
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
EGFR (Phospho-Thr678) Antibody detects endogenous levels of EGFR only when phosphorylated at Thr678.