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c-PLA2 (Phospho-Ser505) Antibody

Catalog No : A0065
  • Applications
    WB, IHC, IF, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-c-PLA2 (Phospho-Ser505) polyclonal Antibody detects c-PLA2 only when phosphorylated at Ser505. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    c-PLA2 (Phospho-Ser505) Antibody
  • Catalog No
    A0065
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:10000
  • Database Links
    Gene ID: 5321, Swiss-Prot #: P47712, OMIM #: 600522, Unigene #: Hs.497200
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human c-PLA2 around the phosphorylation site of Ser505.
  • Immunogen Range
    471-520
  • Modification Sites
    H: S505 M: S505 R: S505
  • MW
    85 kDa
  • NCBI Gene Symbol
    PLA2G4A
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    c-PLA2 (Phospho-Ser505) Antibody detects endogenous levels of c-PLA2 only when phosphorylated at Ser505.
  • Synonyms
    CPLA2, Cytosolic phospholipase A2, PA24, PA24A, PLA2G4, PLA2G4A, Phosphatidylcholine 2-acylhydrolase
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 30', using c-PLA2 (Phospho-Ser505) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of VEC using p-cPLA2 (S505) antibody. Antibody was diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 30', using c-PLA2 (Phospho-Ser505) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using c-PLA2 (Phospho-Ser505) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of VEC using p-cPLA2 (S505) antibody. Antibody was diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.