The ZAP-70 (Phospho-Tyr292) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect ZAP-70 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated ZAP-70 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ZAP-70 phosphorylation.
Details
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Product Name
ZAP-70 (Phospho-Tyr292) Colorimetric Cell-Based ELISA Kit
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Catalog No
CBP1234
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Detection Method
Colorimetric 450 nm
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Dynamic Range
> 5000 Cells
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Storage/Stability
4°C/6 Months
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Reactivity
Human, Mouse, Rat
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Assay Type
Cell-Based
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Database Links
Gene ID: 7535, UniProt ID: P43403, OMIM #: 176947, Unigene #: Hs.234569
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Format
2 x 96-Well Microplates
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Manual (PDF)
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NCBI Gene Symbol
ZAP70
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Sub Type
Phospho
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Synonyms
70 kDa zeta-associated protein, SRK, Syk-related tyrosine kinase, ZA70, ZAP-70, ZAP70, kinase ZAP70
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TargetName
ZAP-70 (Phospho-Tyr292)
Application Images
Western blot analysis of extracts from Jurkat cells treated with UV 15', using ZAP-70 (Phospho-Tyr292) Antibody.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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Western blot analysis of extracts from Jurkat cells treated with UV 15', using ZAP-70 (Phospho-Tyr292) Antibody. |
|
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles. |
|
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits. |
|
The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity. |
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