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YAP (Phospho-Ser127) Antibody

Catalog No : A0757
  • Applications
    WB, IHC, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-YAP (Phospho-Ser127) polyclonal Antibody detects YAP only when phosphorylated at Ser127. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    YAP (Phospho-Ser127) Antibody
  • Catalog No
    A0757
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
  • Database Links
    Gene ID: 10413, Swiss-Prot #: P46937, OMIM #: 606608, Unigene #: Hs.503692
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127.
  • Immunogen Range
    93-142
  • Modification Sites
    H: S127 M: S112 R: S112
  • MW
    48 kDa
  • NCBI Gene Symbol
    YAP1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    YAP (Phospho-Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at Ser127.
  • Synonyms
    65 kDa Yes-associated protein, YAP1, YAP65
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-YAP (S127) Antibody diluted at 1:500
Western Blot analysis of MCF7 cells using Phospho-YAP (S127) Antibody diluted at 1:500
Immunofluorescence analysis of rat-heart tissue. YAP (phospho Ser127) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-heart tissue. YAP (phospho Ser127) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of lysates from COS7 cells treated with HU 2nM 24h, using YAP (Phospho-Ser127) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human heart , using YAP (Phospho-Ser127) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-YAP (S127) Antibody diluted at 1:500
Western Blot analysis of MCF7 cells using Phospho-YAP (S127) Antibody diluted at 1:500
Immunofluorescence analysis of rat-heart tissue. YAP (phospho Ser127) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-heart tissue. YAP (phospho Ser127) Antibody(red) was diluted at 1:200(4°C,ON). Picture A:Target. Picture B: DAPI. Picture C: merge of A+B