Western Blot (WB) - Protocol
Western blot is a commonly used molecular biology technique that analyzes proteins of interest in samples.
Cell lysates are generated from cell culture lysis and separated by weight in gel electrophoresis (SDS-PAGE).
These proteins are then transferred from gel to membranes where they are blocked and probed with specific
antibodies for detection of proteins of interest. With sensitive and specific antibodies, modified proteins
or unmodified proteins are easily detected in small samples.