Anti-Vimentin Antibody detects endogenous levels of total Vimentin protein. Validated for WB and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
Vimentin Antibody
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Catalog No
B1242
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 ELISA: 1:10000
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Database Links
Gene ID: 7431, Swiss-Prot #: P08670, OMIM #: 193060, Unigene #: Hs.455493/Hs.628678
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Immunogen
The antiserum was produced against synthesized peptide derived from human Vimentin.
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Immunogen Range
56-105
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MW
53 kDa
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NCBI Gene Symbol
VIM
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
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Specificity
Vimentin Antibody detects endogenous levels of total Vimentin protein.
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Synonyms
VIME, vimentin
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Target Modification
Unmodified/Total
Application Images
Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30', using Vimentin Antibody. The lane on the right is blocked with the synthesized peptide.
Western Blot analysis of various cells using Vimentin Antibody.
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Western blot analysis of lysates from 293 cells, treated with EGF 200ng/ml 30', using Vimentin Antibody. The lane on the right is blocked with the synthesized peptide. |
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Western Blot analysis of various cells using Vimentin Antibody. |
References
This product has been referenced in:
- Xiaoxiao Lu Honglan Guo Xi Chen Jian Xiao Yong Zou Wei Wang Qiong Chen Effect of RhoC on the epithelial-mesenchymal transition process induced by TGF-β1 in lung adenocarcinoma cells spandidos Western blot analysis.
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