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Universal Protease Activity Assay Kit - Fluorometric

Catalog No : EZ-0535
  • Size
    500 Assays
  • Price
    $195.00
This fluorometric assay is ideal for routine protease assays to isolate proteases or identify contaminating proteases in samples. A casein conjugate, proven to be a generic substrate for a broad range of proteases, has been heavily labeled with green fluorescent dye. The fluorescence of the intact substrate has therefore been subdued, but with protease-catalyzed hydrolysis, the quenching is relieved and the highly fluorescent dye-labeled short peptides will manifest. The fluorescence intensity is directly proportional to protease activity and can be read with a fluorescence microplate reader with excitation at 490 nm and emission at 525 nm using FITC filter set.
Details
  • Product Name
    Universal Protease Activity Assay Kit - Fluorometric
  • Catalog No
    EZ-0535
  • Detection Method
    Fluorometric
  • Storage/Stability
    -20°, C/3 Months
  • Fluorometric Readout [Ex (nm), Em (nm)]
    490, 525
  • Manual
    Download
  • Tar Name
    Universal Protease Activity
Application Images
The Universal Protease Activity Assay Kit - Fluorometric allows for the detection and quantification of endogenous Universal Protease Activity in cell lysates, sera, and plasma.
The Universal Protease Activity Assay Kit - Fluorometric allows for the detection and quantification of endogenous Universal Protease Activity in cell lysates, sera, and plasma.