Anti-Tyr Hydroxylase (Phospho-Ser19) polyclonal Antibody detects Tyr Hydroxylase only when phosphorylated at Ser19. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
Tyrosine Hydroxylase (Phospho-Ser19) Antibody
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Catalog No
A0037
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, IF, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000
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Database Links
Gene ID: 7054, Swiss-Prot #: P07101, OMIM #: 191290/605407, Unigene #: Hs.435609/Hs.523414
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Immunogen
The antiserum was produced against synthesized peptide derived from human Tyrosine Hydroxylase around the phosphorylation site of Ser19.
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Immunogen Range
8-59
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Modification Sites
H: S18 M: S19 R: S19
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MW
58 kDa
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NCBI Gene Symbol
TH
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
Tyr Hydroxylase (Phospho-Ser19) Antibody detects endogenous levels of Tyr Hydroxylase only when phosphorylated at Ser19.
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Synonyms
TH isoform 3, TH isoform a, TH-4, TY3H, TYH, Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase, tyrosine hydroxylase
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 30', using Tyrosine Hydroxylase (Phospho-Ser19) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Tyrosine Hydroxylase (Phospho-Ser19) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using Tyrosine Hydroxylase (Phospho-Ser19) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-TH (S19) Antibody diluted at 1:1000
Western Blot analysis of 293 cells using Phospho-TH (S19) Antibody diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from 293 cells treated with Insulin 0.01U/ml 30', using Tyrosine Hydroxylase (Phospho-Ser19) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human brain, using Tyrosine Hydroxylase (Phospho-Ser19) Antibody. The picture on the right is blocked with the phospho peptide. |
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Immunofluorescence analysis of HUVEC cells, using Tyrosine Hydroxylase (Phospho-Ser19) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-TH (S19) Antibody diluted at 1:1000 |
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Western Blot analysis of 293 cells using Phospho-TH (S19) Antibody diluted at 1:1000 |
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
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