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Thiol Assay Kit - Fluorometric

Catalog No : EZ-0527
  • Size
    200 Assays
  • Price
    $195.00
This assay uses an ultrasensitive fluorometric method to quantify thiol content on a small molecule or on a protein. In the assay, a proprietary non-fluorescent dye reacts with thiol to produce a highly fluorescent adduct. The resulting spectra of the fluorescent adduct is pH-independent and no separation step is necessary. Signals increase with increasing thiol levels, and can be monitored with fluorescence microplate reader with excitation at 490 nm and emission at 520 nm.
Details
  • Product Name
    Thiol Assay Kit - Fluorometric
  • Catalog No
    EZ-0527
  • Detection Method
    Fluorometric
  • Storage/Stability
    -20°, C/3 Months
  • Fluorometric Readout [Ex (nm), Em (nm)]
    490, 520
  • Manual
    Download
  • Range Detection
    ?10 nM
  • Tar Name
    Thiol
Application Images
The Thiol Assay Kit - Fluorometric allows for the detection and quantification of endogenous Thiol within the range of ≥10 nM in cell lysates, sera, and plasma.
The Thiol Assay Kit - Fluorometric allows for the detection and quantification of endogenous Thiol within the range of ≥10 nM in cell lysates, sera, and plasma.