The Tfact Elk1 (Phospho-Ser389) DNA-Binding ELISA Kit components necessary for detection and qualitative analysis of Elk1 (Phospho-Ser389) active transcription factors in eukaryotic nuclear or cell lysates. Tfact ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.
Details
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Product Name
TFact Elk1 (Phospho-Ser389) DNA-Binding ELISA
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Catalog No
TFE-7047
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Detection Method
Colorimetric 450 nm
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Storage/Stability
4°C/6 Months
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Reactivity
Human, Mouse
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Assay Type
DNA-Binding ELISA
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Database Links
Gene ID: 2002, UniProt ID: P19419, OMIM #: 311040, Unigene #: Hs.181128
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Format
12 x 8-Well Microstrips
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Image Legend
The TFact™, Elk1 (Phospho-Ser389) DNA-Binding ELISA detects active Elk1 (Phospho-Ser389) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The DNA-transcription factor complex is treated with or without the stablization buffer.
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Manual (PDF)
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NCBI Gene Symbol
ELK1
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Specificity
The TFact™, Elk1 (Phospho-Ser389) DNA-Binding ELISA Kit detects endogenous levels of Elk1 only when phosphorylated at Ser389.
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Sub Type
Phospho
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Synonyms
ELK1, ETS-domain protein ELK-1, ETS-domain protein Elk-1
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TargetName
Elk1 (Phospho-Ser389)
Application Images
The Tfact Elk1 (Phospho-Ser389) DNA-Binding ELISA detects active Elk1 (Phospho-Ser389) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The DNA-transcription factor complex is treated with or without the stablization buffer.
An illustrated breakdown of Assay Biotechnology's Colorimetric Transcription Factor ELISA assay principles.
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The Tfact Elk1 (Phospho-Ser389) DNA-Binding ELISA detects active Elk1 (Phospho-Ser389) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The DNA-transcription factor complex is treated with or without the stablization buffer. |
|
An illustrated breakdown of Assay Biotechnology's Colorimetric Transcription Factor ELISA assay principles. |
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