Anti-TIF-IA (Phospho-Ser649) polyclonal Antibody detects TIF-IA only when phosphorylated at Ser649. Validated for WB, IHC and ELISA. Reactive for Human. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
TIF-IA (Phospho-Ser649) Antibody
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Catalog No
A8433
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000
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Database Links
Gene ID: 54700, Swiss-Prot #: Q9NYV6, OMIM #: 605121, Unigene #: Hs.460078
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Immunogen
The antiserum was produced against synthesized peptide derived from human TIF-IA around the phosphorylation site of Ser649.
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Immunogen Range
602-651
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Modification Sites
H: S649
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MW
74 kDa
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NCBI Gene Symbol
RRN3
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
TIF-IA (Phospho-Ser649) Antibody detects endogenous levels of TIF-IA only when phosphorylated at Ser649.
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Synonyms
RNA polymerase I-specific transcription initiation factor RRN3, RRN3, transcription initiation factor IA
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Target Modification
Phospho
Application Images
Immunohistochemistry analysis of paraffin-embedded human brain, using TIF-IA (Phospho-Ser649) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from Jurkat cells treated with starved 24h, using TIF-IA (Phospho-Ser649) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain, using TIF-IA (Phospho-Ser649) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western blot analysis of lysates from Jurkat cells treated with starved 24h, using TIF-IA (Phospho-Ser649) Antibody. The lane on the right is blocked with the phospho peptide. |
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