|In the assay, a
fluorogenic probe can be used to indirectly quantify phosphocholine
production from the hydrolysis of sphingomyelin by sphingomyelinase (SMase).
Fluorescence intensity of the resulting red fluorescent product is directly
proportional to phosphocholine formation, and, in turn, SMase activity.
Readings can be made using a fluorescent microplate reader with excitation at
540 and emission at 590 nm or with an absorption microplate reader at 570 nm
for measurements of SMase activity in blood, cell extracts, or other