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Sphingomyelin Asay Kit - Fluorometric

Catalog No : EZ-0526
  • Size
    200 Assays
  • Price
    $195.00
In the assay, a fluorogenic probe can be used to indirectly quantify phosphocholine production from the hydrolysis of sphingomyelin by sphingomyelinase (SMase). Fluorescence intensity of the resulting red fluorescent product is directly proportional to phosphocholine formation, and, in turn, SMase activity. Readings can be made using a fluorescent microplate reader with excitation at 540 and emission at 590 nm or with an absorption microplate reader at 570 nm for measurements of SMase activity in blood, cell extracts, or other solutions. 
Details
  • Product Name
    Sphingomyelin Asay Kit - Fluorometric
  • Catalog No
    EZ-0526
  • Detection Method
    Fluorometric
  • Storage/Stability
    -20°, C/3 Months
  • Fluorometric Readout [Ex (nm), Em (nm)]
    540, 590
  • Manual
    Download
  • Range Detection
    ?1 uM
  • Tar Name
    Sphingomyelin Asay Kit - Fluorometric
Application Images
The Sphingomyelin Asay Kit - Fluorometric allows for the detection and quantification of endogenous Sphingomyelin within the range of ≥1 uM in cell lysates, sera, and plasma.
The Sphingomyelin Asay Kit - Fluorometric allows for the detection and quantification of endogenous Sphingomyelin within the range of ≥1 uM in cell lysates, sera, and plasma.