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Smad3 (Phospho-Ser213) Antibody

Catalog No : A1007
  • Applications
  • Reactivity
    Human, Mouse, Rat
  • Size
  • Price
Anti-Smad3 (Phospho-Ser213) polyclonal Antibody detects Smad3 only when phosphorylated at Ser213. Validated for WB and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
  • Product Name
    Smad3 (Phospho-Ser213) Antibody
  • Catalog No
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
  • Clonality
  • Host Species
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 ELISA: 1:5000
  • Database Links
    Gene ID: 4088, Swiss-Prot #: P84022, OMIM #: 114500/603109, Unigene #: Hs.618504/Hs.714621
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser213.
  • Immunogen Range
  • Modification Sites
    H: S213 M: S213 R: S213
  • MW
    48 kDa
  • NCBI Gene Symbol
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    Smad3 (Phospho-Ser213) Antibody detects endogenous levels of Smad3 only when phosphorylated at Ser213.
  • Synonyms
    JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3
  • Target Modification
Application Images
Image 1 Western blot analysis of lysates from HT29 cells, using Smad3 (Phospho-Ser213) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Western blot analysis of various lysate, antibody was diluted at 1:1000.
This product has been referenced in:
  • "SatoshiKameshimaMuneyoshiOkadaShiroIkedaYukiWatanabeHideyukiYamawaki " "Coordination of changes in expression and phosphorylation of eukaryotic elongation factor 2 (eEF2) and eEF2 kinase in hypertrophied cardiomyocytes " sciencedirect Primary Antibody Read more >
  • "Yafeng Li‡, Delu Song‡, Ying Song‡, Liangliang Zhao‡1, Natalie Wolkow‡2, John W. Tobias§, Wenc "Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)- Signaling in the Retinal Pigment Epithelium*□S " THE JOURNAL OF BIOLOGICAL CHEMISTRY Primary Antibody VOL. 290, NO. 19, pp. 11918–11934, May 8, 2015 DOI 10.1074/jbc.M115.645903 Read more >