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Smad3 (Phospho-Ser208) Antibody

Catalog No : A1005
  • Applications
    WB, IHC, ELISA
  • Reactivity
    Human, Mouse, Rat
  • Size
    50ul
  • Price
    $195.00
Anti-Smad3 (Phospho-Ser208) polyclonal Antibody detects Smad3 only when phosphorylated at Ser208. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    Smad3 (Phospho-Ser208) Antibody
  • Catalog No
    A1005
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
  • Database Links
    Gene ID: 4088, Swiss-Prot #: P84022, OMIM #: 114500/603109, Unigene #: Hs.618504/Hs.714621
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser208.
  • Immunogen Range
    174-223
  • Modification Sites
    H: S208 M: S208 R: S208
  • MW
    48 kDa
  • NCBI Gene Symbol
    SMAD3
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    Smad3 (Phospho-Ser208) Antibody detects endogenous levels of Smad3 only when phosphorylated at Ser208.
  • Synonyms
    JV15-2, MAD-3, MADH3, Mad3, Mothers against DPP homolog 3, Mothers against decapentaplegic homolog 3, SMAD 3, Smad 3
  • Target Modification
    Phospho
Application Images
Image 1 Western blot analysis of lysates from Jurkat cells, using Smad3 (Phospho-Ser208) Antibody. The lane on the right is blocked with the phospho peptide.
Image 2 Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Smad3 (Phospho-Ser208) Antibody. The picture on the right is blocked with the phospho peptide.
Image 3 Western Blot analysis of various cells using Phospho-Smad3 (S208) Antibody
Image 4 Western Blot analysis of Jurkat cells using Phospho-Smad3 (S208) Antibody
References
This product has been referenced in:
  • Jin M1, Lee J2, Lee KY3, Jin Z1, Pak JH4, Kim HS3. "Alteration of TGF-β-ALK-Smad signaling in hyperoxia-induced bronchopulmonary dysplasia model of newborn rats" creative Deed Primary Antibody Read more >