Anti-STAT5B (Phospho-Ser731) polyclonal Antibody detects STAT5B only when phosphorylated at Ser731. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
STAT5B (Phospho-Ser731) Antibody
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Catalog No
A0090
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
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Database Links
Gene ID: 6777, Swiss-Prot #: P51692, OMIM #: 245590/604260, Unigene #: Hs.714309
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Immunogen
The antiserum was produced against synthesized peptide derived from human STAT5B around the phosphorylation site of Ser731.
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Immunogen Range
697-746
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Modification Sites
H: S731 M: S730 R: S730
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MW
89 kDa
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NCBI Gene Symbol
STAT5B
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
STAT5B (Phospho-Ser731) Antibody detects endogenous levels of STAT5B only when phosphorylated at Ser731.
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Synonyms
Signal transducer and activator of transcription 5B
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using STAT5B (Phospho-Ser731) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT5B (Phospho-Ser731) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Stat5 (S726/731) Antibody diluted at 1:500
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Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using STAT5B (Phospho-Ser731) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT5B (Phospho-Ser731) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-Stat5 (S726/731) Antibody diluted at 1:500 |
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