Anti-SRF (Phospho-Ser99) polyclonal Antibody detects SRF only when phosphorylated at Ser99. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
SRF (Phospho-Ser99) Antibody
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Catalog No
A0088
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse, Rat
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000
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Database Links
Gene ID: 6722, Swiss-Prot #: P11831, OMIM #: 600589, Unigene #: Hs.520140
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Immunogen
The antiserum was produced against synthesized peptide derived from human SRF around the phosphorylation site of Ser99.
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Immunogen Range
71-120
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Modification Sites
H: S103 M: S99 R: S99
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MW
51 kDa
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NCBI Gene Symbol
SRF
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
SRF (Phospho-Ser99) Antibody detects endogenous levels of SRF only when phosphorylated at Ser99.
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Synonyms
serum response factor
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from LOVO cells treated with Serum 10% 15', using SRF (Phospho-Ser99) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using SRF (Phospho-Ser99) Antibody. The picture on the right is blocked with the phospho peptide.
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Western blot analysis of lysates from LOVO cells treated with Serum 10% 15', using SRF (Phospho-Ser99) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using SRF (Phospho-Ser99) Antibody. The picture on the right is blocked with the phospho peptide. |
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