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> Products > ELISA Kits > Fluorometric Cell-Based Phospho ELISA Kits

SHP-2 (Phospho-Tyr542) Fluorometric Cell-Based ELISA Kit

Datasheet SDS Protocol Booklet
Catalog No : FLUO-CBP1026
  • Reactivity
    Human,
  • Reactivity
    Mouse,
  • Reactivity
    Rat
  • Assay Type
    Cell-Based ELISA
  • Size
    1 Kit
  • Price
    $480.00
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The SHP-2 (Phospho-Tyr542) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SHP-2 (Phospho-Tyr542) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated SHP-2 (Phospho-Tyr542) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on PTPN11 phosphorylation.
Details
  • Product Name
    SHP-2 (Phospho-Tyr542) Fluorometric Cell-Based ELISA Kit
  • Catalog No
    FLUO-CBP1026
  • Detection Method
    Fluorometric
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 5781, UniProt ID: Q06124, OMIM #: 151100/163950/163955/176876/607785, Unigene #: Hs.506852/Hs.646231
  • Format
    Two 96-Well Plates
  • Manual (PDF)
    Download
  • NCBI Gene Symbol
    PTPN11
  • Sub Type
    Phospho
  • Synonyms
    PTN11, PTP-1D, PTP-2C, PTP2C, PTPN11, Protein-tyrosine phosphatase 2C, Protein-tyrosine phosphatase, non-receptor type 11, SH-PTP2, SH-PTP3, SHPTP2
  • TargetName
    Phospho-SHP-2 (Tyr542)
Application Images
Image 1 Western blot analysis of extracts from A431 cells, using SHP-2 (Phospho-Tyr542) Antibody.
Image 2 HeLa cells were starved for 24 hrs. and stimulated with 1mM Vanadate for 1 hr.
Image 3 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 4 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 5 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.
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