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SHP-1 (Phospho-Tyr536) Fluorometric Cell-Based ELISA Kit

Catalog No : FLUO-CBP1025
  • Reactivity
  • Reactivity
  • Reactivity
  • Assay Type
    Cell-Based ELISA
  • Size
    1 Kit
  • Price
The SHP-1 (Phospho-Tyr536) Fluorometric Cell-Based Phospho ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SHP-1 (Phospho-Tyr536) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated SHP-1 (Phospho-Tyr536) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on PTPN6 phosphorylation.
  • Product Name
    SHP-1 (Phospho-Tyr536) Fluorometric Cell-Based ELISA Kit
  • Catalog No
  • Detection Method
  • Dynamic Range
    > 5000 Cells
  • Storage/Stability
    4°C/6 Months
  • Reactivity
    Human, Mouse, Rat
  • Assay Type
    Cell-Based ELISA
  • Database Links
    Gene ID: 5777, UniProt ID: P29350, OMIM #: 176883, Unigene #: Hs.63489
  • Format
    Two 96-Well Plates
  • Manual (PDF)
  • NCBI Gene Symbol
  • Sub Type
  • Synonyms
    70Z-SHP, HCP, HCPH, Hematopoietic cell protein-tyrosine phosphatase, PTN6, PTP-1C, PTP1C, PTPN6, Protein-tyrosine phosphatase 1C, Protein-tyrosine phosphatase SHP-1, Protein-tyrosine phosphatase, non-receptor type 6, SH-PTP1
  • TargetName
    Phospho-SHP-1 (Tyr536)
Application Images
Image 1 Western blot analysis of extracts from RAW264.7 cells treated with EGF 200ng/ml 5', using SHP-1 (Phospho-Tyr536) Antibody.
Image 2 Hek 293 were starved for 24 hrs. and stimulated with 10% Serum for 2 hrs.
Image 3 An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Image 4 A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Image 5 The Anti-GAPDH Antibody is a mouse monoclonal antibody used as a positive control in Assay Biotechnology's Cell-Based ELISA kits. It was tested on Western Blots with the tissue lysates from human, mouse, and rat for specificity.