Anti-SGK (Phospho-Ser422) polyclonal Antibody detects SGK only when phosphorylated at Ser422. Validated for WB, IHC and ELISA. Reactive for Human, Mouse, Rat. AssayBiotechnology an original antibody manufacturer.
Details
-
Product Name
SGK (Phospho-Ser422) Antibody
-
Catalog No
A0087
-
Storage/Stability
Stable at -20°C for at least 1 year.
-
Applications
WB, IHC, ELISA
-
Clonality
Polyclonal
-
Host Species
Rabbit
-
Reactivity
Human, Mouse, Rat
-
Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
-
Database Links
Gene ID: 6446, Swiss-Prot #: O00141, OMIM #: 602958, Unigene #: Hs.510078
-
Immunogen
The antiserum was produced against synthesized peptide derived from human SGK around the phosphorylation site of Ser422.
-
Immunogen Range
381-430
-
Modification Sites
H: S422 M: S422 R: S421
-
MW
48 kDa
-
NCBI Gene Symbol
SGK1
-
Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
-
Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
-
Specificity
SGK (Phospho-Ser422) Antibody detects endogenous levels of SGK only when phosphorylated at Ser422.
-
Synonyms
SGK1, SERUM/GLUCOCORTICOID-REGULATED KINASE
-
Target Modification
Phospho
Application Images
Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of VEC HY926 cells using Phospho-SGK1 (S422) Antibody diluted at 1:500
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide.
|
Western blot analysis of lysates from HeLa cells treated with Insulin 0.01U/ml 15', using SGK (Phospho-Ser422) Antibody. The lane on the right is blocked with the phospho peptide. |
|
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using SGK (Phospho-Ser422) Antibody. The picture on the right is blocked with the phospho peptide. |
|
Western Blot analysis of VEC HY926 cells using Phospho-SGK1 (S422) Antibody diluted at 1:500 |
|
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4°,ON). Negative control (right) obtaned from antibody was pre-absorbed by immunogen peptide. |
References
This product has been referenced in:
- Sherry A. Weppler, Amelia Stegeman, Corinna Warburton, Dita Strutt, Dana Masin, Marcel B. Bally Combined RNAi-Mediated Suppression of Rictor and EGFR Resulted in Complete Tumor Regression in an Orthotopic Glioblastoma Tumor Model PLOS One Primary Antibody
Read more >
Follow us on Twitter
Follow us on Linkedin
Like us on Facebook
Subscribe to our Channel