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SEPARASE (Phospho-Ser801) Antibody

Catalog No : A0086
  • Applications
    WB, IHC, IF, ELISA
  • Reactivity
    Human, Mouse
  • Size
    50ul
  • Price
    $195.00
Anti-SEPARASE (Phospho-Ser801) polyclonal Antibody detects SEPARASE only when phosphorylated at Ser801. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse. AssayBiotechnology an original antibody manufacturer.
Details
  • Product Name
    SEPARASE (Phospho-Ser801) Antibody
  • Catalog No
    A0086
  • Storage/Stability
    Stable at -20°C for at least 1 year.
  • Applications
    WB, IHC, IF, ELISA
  • Clonality
    Polyclonal
  • Host Species
    Rabbit
  • Reactivity
    Human, Mouse
  • Application Dilutions
    WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
  • Database Links
    Gene ID: 9700, Swiss-Prot #: Q14674, OMIM #: 600937/601820/606176/610582, Unigene #: Hs.248141
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human SEPARASE around the phosphorylation site of Ser801.
  • Immunogen Range
    767-816
  • Modification Sites
    H: S801 M: S1189
  • MW
    233 kDa
  • NCBI Gene Symbol
    ESPL1
  • Physical Form
    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Purification
    The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
  • Specificity
    SEPARASE (Phospho-Ser801) Antibody detects endogenous levels of SEPARASE only when phosphorylated at Ser801.
  • Synonyms
    ESP1, SEPARIN, EXTRA SPINDLE POLES-LIKE 1, extra spindle poles like 1 (S. cerevisiae)
  • Target Modification
    Phospho
Application Images
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Separase (S801) Antibody diluted at 1:1000
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Separase (S801) Antibody diluted at 1:1000