Anti-SEPARASE (Phospho-Ser801) polyclonal Antibody detects SEPARASE only when phosphorylated at Ser801. Validated for WB, IHC, IF and ELISA. Reactive for Human, Mouse. AssayBiotechnology an original antibody manufacturer.
Details
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Product Name
SEPARASE (Phospho-Ser801) Antibody
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Catalog No
A0086
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Storage/Stability
Stable at -20°C for at least 1 year.
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Applications
WB, IHC, IF, ELISA
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Clonality
Polyclonal
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Host Species
Rabbit
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Reactivity
Human, Mouse
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Application Dilutions
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
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Database Links
Gene ID: 9700, Swiss-Prot #: Q14674, OMIM #: 600937/601820/606176/610582, Unigene #: Hs.248141
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Immunogen
The antiserum was produced against synthesized peptide derived from human SEPARASE around the phosphorylation site of Ser801.
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Immunogen Range
767-816
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Modification Sites
H: S801 M: S1189
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MW
233 kDa
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NCBI Gene Symbol
ESPL1
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Physical Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Purification
The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Specificity
SEPARASE (Phospho-Ser801) Antibody detects endogenous levels of SEPARASE only when phosphorylated at Ser801.
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Synonyms
ESP1, SEPARIN, EXTRA SPINDLE POLES-LIKE 1, extra spindle poles like 1 (S. cerevisiae)
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Target Modification
Phospho
Application Images
Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide.
Western Blot analysis of various cells using Phospho-Separase (S801) Antibody diluted at 1:1000
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Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using SEPARASE (Phospho-Ser801) Antibody. The lane on the right is blocked with the phospho peptide. |
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide. |
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Immunofluorescence analysis of HUVEC cells, using SEPARASE (Phospho-Ser801) Antibody. The picture on the right is blocked with the phospho peptide. |
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Western Blot analysis of various cells using Phospho-Separase (S801) Antibody diluted at 1:1000 |
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